CUSTOMIZED SERVICES

THE PIONEER OF "ONE-STOP" SCIENTIFIC RESEARCH SERVICES FOR PHASE SEPARATION OF BIOMOLECULES IN CHINA

TOP EXPERT TEAM

Multiple Nature/Mol Cell paper achievements

HIGH END EXPERIMENTAL PLATFORM

Zeiss LSM980 confocal microscope, fluorescence inverted microscope, cell culture system

STRICT QUALITY CONTROL

Multi concentration titration, strictly distinguishing between true phase separation and artifacts

ONE-STOP SERVICE

From genes → proteins → cells → functions, complete experimental chain

Customized Services

Phase Separation Biotechnology (Wuhan) Co., Ltd. specializes in cutting-edge technology research and development, professional technical services, and related product development in the field of biological macromolecule phase separation (LLPS). We are committed to providing global life science research institutions and biopharmaceutical companies with a full-chain, high-precision solution from phase separation mechanism exploration, experimental verification, high-throughput screening to related biomolecule and consumable development, promoting the application and development of phase separation technology in basic research, disease mechanism analysis, new drug target discovery, and innovative drug research and development.

Intracellular Experiments

Intracellular Experiments

· Confirmation of intracellular phase separation
· Dynamic characterization of intracellular phase separation - FRAP
· Dynamic characterization of intracellular phase separation - Fusion
· Functional validation of key domains/IDRs in cells
· Study of phase separation properties of pathogenic mutants in cells
· Validation of phase separation potential using optogenetic Cry2 system

In Vitro Experiments

In Vitro Experiments

· Confirmation of phase separation formation in vitro
· Dynamic characterization of phase separation in vitro - FRAP
· Dynamic characterization of phase separation in vitro - Fusion
· Functional validation of key domains/IDRs in vitro
· Study of phase separation properties of pathogenic mutants in vitro
· Validation of phase separation potential using optogenetic Cry2 system

Personalized phase separation research plan design

Personalized phase separation research plan design

High-throughput phase separation potential screening (b-isox-MS)

High-throughput phase separation potential screening (b-isox-MS)

Proximity-dependent phase separation interaction network analysis (TurboID-MS)

Proximity-dependent phase separation interaction network analysis (TurboID-MS)

Stable Cell Lines

Stable Cell Lines

· Construction of full-length/truncated protein overexpression stable cell lines
· Construction of endogenous tag N/C-terminal knock-in stable cell lines
· Construction of full-length/truncated gene knockout stable cell lines

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